The Ettan IPGphor Isoelectric Focusing System performs first dimension isoelectric focusing (IEF) in 2-dimensional (2-D) electrophoresis.
The system comprises Immobiline DryStrip gel strips, which contain an immobilized pH gradient (IPG); strip holders, which serve as both rehydration and IEF chambers for the IPG strips; and the Ettan IPGphor unit, which includes a high-voltage DC power supply, solid state temperature control, and can be programmed with up to ten user-defined IEF protocols. Programmable functions include:
• rehydration time
• platform temperature
• maximum current limit
• voltage change pattern
• voltage for each step in the isoelectric focusing program, and step duration.
Working surface
Electrode areas: Gold-plated copper
Strip holder capacity: Up to 12 fixed-length, up to 9 cup loading
Platform temperature: 18°C – 25°C, ±1°C
Strip holders
Material: Aluminum oxide ceramic base, acrylic cover, polycarbonate, platinum on titanium
Holders for IPG strip lengths: 70, 110, 130, 180, and 240 mm
User interface
Control panel: 7-key membrane keypad.
Liquid crystal display (LCD): 2 lines, 16 characters per line
Programmable parameters: Rehydration time, platform temperature, maximum current limit per strip, step voltage, step voltage change pattern, and step duration.
Protocol capacity: 10, with up to 9 steps each
Power outputs
Voltage: 0 – 8000 V, resolution 10 V
Current 0 – 1.5 mA, resolution 0.01 mA
Power: 12 W max
Physical parameters
Dimensions (HxWxD): 140 x 250 x 460 mm
Weight: 6.8 kg